Fig. 6

TPLR downregulated TGF-β/Smad signaling pathway in mice. A The expressions of Smad4, Smad2/3 and Smad7 in liver with or without TPLR treatment by western blotting. B Quantitative analysis for western blotting of Smad2/3, Smad4 and Smad7 (n = 3). C Dual immunofluorescence staining for TGF-β1 (green) and α-SMA (red) in mice liver tissues with or without TPLR treatment. scale bar, 20 μm. D Quantitative analysis of C. E Dual immunofluorescence staining for Col1α1(green) and FN (red) in mice liver tissues with or without TPLR treatment. scale bar, 20 μm. F Quantitative analysis of E. Images are representative of 3 mice per group. G The mRNA levels of TGF-β1, α-SMA, Col1α1 and FN with or without TPLR treatment by qPCR (n = 6). ^###P < 0.001, ^####P < 0.0001 vs. normal group; ^*P < 0.05, ^**P < 0.01, vs. model group