Fig. 6

Effect on the viability and protein expression of H9c2 cells in hypoxia test. a Control group; b Model group; c RAF group (0.63 μg/mL); d RAF group (1.25 μg/mL); e RAF group (2.5 μg/mL); f RFA group (5 μg/mL). After treatment with RAF or vehicle control for 8 h, the viability of H9c2 cells was examined by A Morphological observations (images magnification at 10 ×) and B Hoechst 33258 fluorescence detection (images magnification at 40 ×). C After treatment with RAF (1.25, 2.5, and 5 μg/mL) or vehicle control for 8 h, the protein expression of p-p38 MAPK, p-MKK3 and Bax/Bcl-2 was analyzed by western blot. ^*P < 0.05, ^**P < 0.01 compared with the Mod group