Fig. 1

LQ has significant anti-inflammatory effects in vitro and in vivo. A The chemical structure of LQ. B LQ improved the survival rate of LPS-microinjected zebrafish larvae. One hundred and eighty larvae were randomly divided into 6 groups (n = 30). larvae were exposed to indicated dosages of LQ after PBS or LPS (2 mg/mL) yolks microinjection, then the mortalities of the larvae were observed for 72 h. C Representative fluorescence images and (D) statistical analysis of macrophages in yolk were shown. E RAW264.7 cells viability was determined by MTT assay. F RAW264.7 macrophage cells were pretreated with LQ (100, 200, 300 µM) for 2 h, then LPS (100 ng/mL) were added for another 22 h incubation. The supernatants were collected and the concentrations of TNF-α and IL-6 were determined by ELISA. Data were presented as mean ± SEM, n = 3, ^###P < 0.001 versus control; *P < 0.05 versus LPS, **P < 0.01 versus LPS, ***P < 0.001 versus LPS. One-way ANOVA, post hoc comparisons, Turkey’s test