Fig. 2

LQ exerts anti-inflammatory effects through inhibiting JNK. A LQ inhibited the phosphorylation of JNK. RAW264.7 macrophage cells were treated with LQ (100–300 µM) for 2 h, then LPS (100 ng/mL) were treated for another 22 h. Protein expression levels of p-JNK, JNK, p-ERK, ERK, p-p38 and p38 were determined by Western blotting. B LQ inhibits the pro-inflammatory cytokines by suppressing of JNK. RAW264.7 cells were treated with JNK inhibitor SP600125 (70 nM) or JNK activator anisomycin (18 nM) for 2 h before the administration of LQ, and treated with LPS for another 22 h. Cell supernatants were collected, and the concentration of TNF-α and IL-6 were measured by ELISA. Data are presented as mean ± SEM, n = 3, ^*P < 0.05, ^***P < 0.001 versus LQ group. One-way ANOVA, post hoc comparisons, Turkey’s test. Columns, means; error bars, SEM