Fig. 4

LQ inhibits Nur77/c-Jun by JNK suppression. A RAW264.7 cells were pretreated with LQ, SP600125 (8 µM) or Anisomycin (18 nM) for 2 h and then incubated with LPS (100 ng/mL) for another 22 h. Western blotting was employed to detect the expression levels of p-Nur77^Ser351, Nur77, p-c-Jun, and c-Jun^Ser63. B JNK is involved in LQ-induced inhibition of the Nur77/c-Jun pathway. RAW264.7 cells were transfected with JNK siRNA for 24 h, and then treated with LQ 2 h, followed by LPS stimulation for another 22 h. The protein levels of p-Nur77 (Ser351), Nur77, p-c-Jun (Ser63), c-Jun were determined. C JNK overexpression inhibited the luciferase activity of Nur77. HEK293 T cells were co-transfected with JNK overexpression plasmid, Nur77-Luc and TK-Luc plasmids for 24 h. Then, cells were incubated with LQ (300 µM) for 24 h. The luciferase activity of Nur77 was determined using a Dual-Glo luciferase assay system kit. Data are presented as mean ± SEM, ^***P < 0.001 versus Nur77-luc group, ^##P < 0.01 versus JNK overexpress group. One-way ANOVA, post hoc comparisons, Turkey’s test. Columns, means; error bars, SEM