Fig. 5

LQ decreases c-Jun expression level and DNA binding activity by the up-regulation of Nur77. RAW264.7 cells were transfected with Nur77 siRNA for 24 h, after that, cells were treated with LQ for 2 h and then stimulated with LPS for another 22 h. The total protein and nuclear proteins were extracted. A Expression levels of p-c-Jun (Ser63) and c-Jun were determined by Western blotting. B The c-Jun DNA binding activity was examined by EMSA