Fig. 6

LQ inhibits the activation of the JNK/Nur77/c-Jun pathway in LPS-induced ALI in mice. A Lung tissues were dehydrated, embedded, sliced, H&E stained and photographed (40 ×). (B − E) Anisomycin counteracts the decrease of the inflammation and edema induced by LQ in ALI mice. At the end of experiment, lungs were separated and weighed to get the W/D ratio (B). BALF was obtained to detect the total protein concentration by the BCA protein assay kit (C). The levels of TNF-α and IL-6 (D) were evaluated by ELISA. E The lung tissues were homogenized and lysis to obtain total protein. The expression levels of Nur77, p-Nur77 (Ser351), JNK, p-JNK (Thr183/Tyr185), c-Jun, p-c-Jun (Ser63) in total cell lysates were determined by Western blotting. Data were shown as mean ± SEM. ^###P < 0.001 versus control group, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus LPS group; ⁺P < 0.05, ⁺⁺P < 0.01 versus LQ-80 group. one-way ANOVA, post hoc comparisons, Turkey’s test