Fig. 2

PSO inhibits the migration and invasion of OS cells. The effect of PSO on the migration ability of human OS cells was detected by wound healing (a–d, × 100) and Transwell (e–f, crystal violet staining, × 100) assays. The effect of PSO on the invasive ability of human OS cells was detected by Transwell assay (g–h, crystal violet staining, × 100). The effect of PSO on the protein expression of MMP-2, MMP-9, and snail was assessed by western blotting i–l. PSO significantly inhibited the migration and invasion of OS cells (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, vs. the control group, n = 3)