Fig. 4

OS cells were treated with 30 μM PSO for 24 h, and the global changes in gene expression were analyzed by RNA-sequencing. a–b Volcano maps showing differentially expressed genes in OS cells treated with PSO compared to untreated control cells. c Altered signaling pathways in PSO-treated OS cells according to KEGG enrichment analysis. d GSEA enrichment plots for PSO-treated OS cells. e–h Western blotting analysis of FAK, p-FAK, PI3K, p-PI3K, AKT, and p-AKT levels in OS cells treated with PSO (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, vs. the control group, n = 3)