Fig. 5

a Venn diagram of DEGs from selected top five pathways. b Kaplan–Meier curve representing the effects of different genes on the overall survival of OS patients. c–d Protein expression of ITGB1, PIK3CD, MAPK3, and PRKCA with or without PSO treatment. e After transfection with 100 nmol/L of the indicated siRNA or NC for 48 h, the expression of ITGB1 mRNA in 143B cells was analyzed by real-time PCR. f–g After transfection of siRNA into 143B cells, the expression of FAK, p-FAK, Akt and p-Akt pathway proteins was analyzed by western blot. h 2D and 3D molecular structures of PSO, stable complex, and docking pocket of PSO with ITGB1. (i) Relative expression of ITGB1 mRNA after PSO treatment (**P < 0.01, ****P < 0.0001, vs. the control group, n = 3)