Fig. 10

MON inhibited the activation of NF-κB signaling pathway in DMM mice. A, B Immunofluorescence staining showing the phosphorylation of IκBα (green) and the nuclear translocation of p65 (green) in mice knee joints at 56 days after MON treatment. C, D Western blot analysis and relative quantification showing the expression levels of NF-κB signaling pathway related proteins (p-IκBα, IκBα, p-p65 and p65) in DMM mice at 56 days after MON treatment. All experiments were performed in triplicated and data were presented as the mean ± SD, n = 3 per group. *P < 0. 05, **P < 0. 01