Fig. 3

MON inhibited IL-1β-induced pyroptosis in chondrocytes. A, B Immunofluorescence staining of NLRP3 (green) and GSDMD (red) were performed respectively in IL-1β-induced chondrocytes after MON treatment. C, D Western blot analysis and relative quantification showing the expression levels of pyroptosis related proteins (NLRP3, ASC, Cleaved caspase-1 and GSDMD) in IL-1β-induced chondrocytes after MON treatment. All experiments were performed in triplicated and data were presented as the mean ± SD, n = 3 per group. ^##P < 0. 01 versus control group;*P < 0. 05, **P < 0. 01 versus IL-1β group