Fig. 4

MON inhibited the NF-κB signaling pathway in chondrocytes. A, B Immunofluorescence staining showing the phosphorylation of IκBα (green) and the nuclear translocation of p65 (red) in IL-1β-induced chondrocytes after MON treatment. C, D Western blot analysis and relative quantification showing the expression levels of NF-κB signaling pathway related proteins (p-IκBα, IκBα, p-p65 and p65) in IL-1β-induced chondrocytes after MON treatment. All experiments were performed in triplicated and data were presented as the mean ± SD, n = 3 per group. ^##P < 0. 01 versus control group;*P < 0. 05, **P < 0. 01 versus IL-1β group