Fig. 5

The protective effects of MON are mediated through the inactivation of NF-κB signaling. A–C Western blot analysis and relative quantification showing the expression levels of cartilage matrix degradation related proteins (MMP3, MMP13, Collagen II and SOX9), apoptosis related proteins (Bcl-2, Bax and Cleaved caspase-3) and pyroptosis related proteins (NLRP3, ASC, Cleaved caspase-1 and GSDMD) in IL-1β-induced chondrocytes after MON and recombinant NF-κB protein treatment. All experiments were performed in triplicated and data were presented as the mean ± SD, n = 3 per group. *P < 0. 05, **P < 0. 01