Fig. 8

MON inhibited chondrocyte apoptosis in DMM mice. A, B TUNEL staining showing the chondrocyte apoptosis and quantitative estimation of the number of TUNEL positive cells in mice knee joints at 56 days after MON treatment. C–E Western blot analysis and relative quantification showing the expression levels of apoptosis related proteins (Bcl-2, Bax and Cleaved caspase-3) in DMM mice at 56 days after MON treatment. All experiments were performed in triplicated and data were presented as the mean ± SD, n = 3 per group. *P < 0. 05, **P < 0. 01