Fig. 9

MON inhibited chondrocyte pyroptosis in DMM mice. A, B Immunofluorescence staining of NLRP3 (green) and GSDMD (red) were performed respectively in mice knee joints at 56 days after MON treatment. C, D The relative quantification analysis of NLRP3 and GSDMD positive cells. E–G Western blot analysis and relative quantification showing the expression levels of pyroptosis related proteins (NLRP3, ASC, Cleaved caspase-1 and GSDMD) in DMM mice at 56 days after MON treatment. All experiments were performed in triplicated and data were presented as the mean ± SD, n = 3 per group. *P < 0. 05, **P < 0. 01