Fig. 4

BGXJW administrations upregulate mRNA and protein levels of key enzymes of β-oxidation reduced by alcohol. C57BL/6 mice were grouped and fed as indicated in material and methods. The mRNA and protein were collected and tested at the indicated time points, respectively. A–D The mRNA levels of CPT1A, PPAR-α, ACOX1, LCAD, in liver tissues were subjected to RT-qPCR analysis. E PPARα, CPT1A in liver lysates were also determined by western blot. All data are expressed as the mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs ctrl group; nsP > 0.05, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs EtOH group. Crtl, contrl; EtOH,ethanol model; BGXJW, Bao-Gan-Xing-Jiu-Wan