Fig. 3
PNS inhibits cardiomyocyte pyroptosis. A H9c2 cellular morphology was observed by electron microscope; scale bar = 50 μm. B Cell viability was determined by CCK-8. C LDH was measured by ELISA. D Cell pyroptosis was detected by PI staining; scale bar = 50 μm. H9c2 cells were incubated in an atmosphere of 94% N2, 5% CO2, and 1% O2 for 4 h and treated with VX765 (a specific pyroptosis inhibitor) and/or 400 μg/mL PNS. **p < 0.01 vs. control group; #p < 0.05, ##p < 0.01 vs. hypoxia group; ^p < 0.05, ^^p < 0.01 vs. hypoxia + PNS group. PNS: Panax notoginseng saponins; CCK-8: cell counting kit-8; LDH: Lactate dehydrogenase; PI: propidium iodide