Fig. 4
PNS inhibits NLRP3 inflammasome-mediated cardiomyocyte pyroptosis. A Protein expression levels of GSDMD-N and caspase-1 were measured by immunofluorescence staining; scale bar = 25 μm. B Western blotting was used to determine protein expression levels of NLRP3, IL-1β, TGF-β1, GSDMD-N, and caspase-1. H9c2 cells were incubated in an atmosphere of 94% N2, 5% CO2, and 1% O2 for 4 h and treated with VX765 and/or 400 μg/mL PNS. **p < 0.01 vs. control group; #p < 0.05, ##p < 0.01 vs. hypoxia group; ^^p < 0.01 vs. hypoxia + PNS group. PNS: Panax notoginseng saponins; TGF: transforming growth factor; IL: interleukin; GSDMD-N: N terminal of gasdermin D