Fig. 4

CAE inhibited TGF-β1-induced EMT in A549 cells. A CCK-8 experiment in A549 treated by a series dosages of CAE. B The protein expression of N-cadherin, α-SMA and E-cadherin were examined by western blots. C–E Quantitative analysis of N-cadherin, α-SMA and E-cadherin expression (n = 3). (F) CHIP assay of Egr1 binding COL1A1 gene promoter with EZH2 binding PTEN promoter as positive control. All data were presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the TGF-β1 group, ^# P < 0.05, ^## P < 0.01, ^### P < 0.001 vs. control group