Fig. 6

CAE reduced EMT through Wnt/β-catenin signal pathway. A The protein expression of β-catenin, Wnt-1, Stat3, P-Stat3, Stat6 and P-Stat6 were examined by western blots. B–E Quantitative analysis of β-catenin, Wnt-1, P-Stat3 and P-Stat6 expression (n = 3). (F) GSK3β and β-catenin immunoprecipitation experiment. G The protein expression of P-β-catenin (Ser33) and P-GSK3β (Ser9) were examined by western blots. (H-I) Quantitative analysis of P-β-catenin (Ser33) and P-GSK3β (Ser9) expression (n = 3). All data were presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the TGF-β1 group, ^# P < 0.05, ^## P < 0.01, ^### P < 0.001 vs. control group