Fig. 1

Z-LIG selectivity induced mitochondrial dysfunction in HL-60 cells. A, B Cell viability was measured using SRB after adriamycin (ADR) treatment (32 nM for HL-60 cells; 2.5 µM for 293T cells) or Z-LIG treatment for 72 h. C, D Cells were pretreated with 2-DG (5 mM) and sodium pyruvate (2 mM) for 1 h and stimulated with Z-LIG for 24 h; then, mitochondrial ATP was detected using luciferase assay. E Apoptosis was quantified using flow cytometry after Z-LIG treatment (48 h). F, G MMP and mitochondrial ROS induced by Z-LIG (24 h) were measured using flow cytometry after staining with the appropriate indicator. Values are presented as the means ± SD (n = 3 per group). *P < 0.05, ** P < 0.01, and *** P < 0.001 vs. CTRL