Fig. 3

Effect of NUR77/NOR1 overexpression on cellular mitochondrial function. A–F Protein samples analyzed by western blotting for NUR77 and NOR1. B and C were densitometric analyses of the western blots shown in A. E and F were densitometric analyses of the western blots shown in D. G, H Cells were transfected with plasmid for 6 h and treated with 2-DG (5 mM) and sodium pyruvate (2 mM) for 24 h; then, mitochondrial ATP was determined using luciferase assay. I, J Cells were transfected with the indicated plasmid. MMP and mitochondrial ROS were measured after staining with the appropriate indicators. Values are presented as the means ± SD (n = 3 per group). *P < 0.05, ** P < 0.01, and *** P < 0.001 vs. Vector