Fig. 4

Effect of NUR77/NOR1 in Z-LIG-induced mitochondrial dysfunction. A Nur77 and Nor1 protein levels induced by Z-LIG (25 µM) at different time points analyzed with western blotting. B, C Densitometric analyses of the western blots in A. D HL-60 cells were transfected with siRNA for 24 h, and NUR77 and NOR1 levels were measured by western blotting. (E, F) Densitometric analyses of the western blots in D. G HL-60 cells were transfected with siRNA for 6 h. Thereafter, the cells were pretreated with 2-DG (5 mM) and sodium pyruvate (2 mM) for 1 h and stimulated with Z-LIG (25 µM) for 24 h. Mitochondrial ATP was detected using luciferase assay. H, I HL-60 cells were transfected with siRNA for 6 h and treated with Z-LIG (25 µM) for 24 h. MMP and mitochondrial ROS were detected after staining with the appropriate indicators. Values are presented as the means ± SD (n = 3 per group). *P < 0.05, ** P < 0.01, and *** P < 0.001 vs. CTRL. ^$$P < 0.01 vs. NC. ^##P < 0.01 vs. NC + Z-LIG. ⁺⁺P < 0.01 vs. siNur77 + Z-LIG. ^{^^}P < 0.01 vs. siNor1 + Z-LIG