Fig. 5

HL-60 ρ0 cells were resistant to NUR77/NOR1 overexpression or Z-LIG treatment. A The whole genome DNA of HL-60 and HL-60 ρ0 cells was isolated for PCR. (1) HL-60 cell expansion; (2) HL-60 ρ0 cell expansion; (3) Molecular standard. B, E Cells were treated with Z-LIG or transfected with the plasmid for 48 h; then, apoptosis was measured using flow cytometry. F–I HL-60 cells were pretreated with uridine (1 mg/mL) and pyruvate (1 mM) for 1 h and stimulated with Z-LIG (25 µM) or transfected with the plasmid for 48 h. Apoptosis was measured using flow cytometry. Values are presented as the means ± SD (n = 3 per group). ** P < 0.01 and *** P < 0.001 vs. indicated group