Fig. 7

Anti-AML activity of Z-LIG in primary AML cells and NOD/SCID mice. A, B Cell viability of primary AML cells measured using SRB after treatment with Z-LIG (72 h). C, D The number of apoptotic cells was measured after treatment with Z-LIG (48 h). E, F Cells were pretreated with 2-DG (5 mM) and sodium pyruvate (2 mM) for 1 h, and after treatment with Z-LIG (25 µM for 24 h), mitochondrial ATP was detected using luciferase assay. G, H Tumor volume and mass (n = 6 per group). (I) COX II, COX IV, MYC, Nur77 and NOR1 levels in the tumor analyzed with western blotting. J Densitometric analyses of the western blots in H. Values are presented as the means ± SD (n = 3 per group). *P < 0.05, ** P < 0.01 and *** P < 0.001 vs. CTRL