Fig. 2
From: Multi-target regulatory mechanism of Yang Xin Tang − a traditional Chinese medicine against dementia
Effects of YXT and its selected herbal components on AChE activity and ChAT expression. A PC12 cell lysates were treated with extracts of YXT and its components at 1 mg/mL for 30 min, followed by an Ellman assay to determine the AChE activity (n = 6). B PC12 cell lysates were treated with ethanol extracts (EtOH) of PA, PS, and aqueous extract (Aq) of RP at various concentrations (100 µg/mL to 1 mg/mL) prior to the Ellman assay (n = 6). C Tacrine was used as a positive control for the Ellman assay, and the AChE activity measured in its absence was defined as 100% (Con). D RDIN cells were treated with the indicated herbal extracts for 7 days and analyzed by Western blotting to determine the amount of ChAT with β-actin as a loading control. The results were compared with basal group (Veh) in the absence of herbal treatment. *P < 0.05 (ANOVA); n = 6