Fig. 6
From: Multi-target regulatory mechanism of Yang Xin Tang − a traditional Chinese medicine against dementia
Effects of YXT and its selected herbal components on CREB and ERK phosphorylation. RDIN culture, SH-SY5Y and U-87 MG cell lines were treated with ethanol herbal extracts for 10 min. Western blotting analysis of (A) P-CREB and (B) P-ERK are shown. β-actin served as a loading control and the amount of phosphoprotein was normalized against total amount of the respective protein as a measure of the phosphorylation activity. Bar graphs illustrate the relative fold change of band intensities detected by anti-phosphoantibodies as compared to the basal group (Veh) detected in the absence of herbal treatment. *P < 0.05 (ANOVA); n = 3