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Table 1 Effects of YXT on the luciferase-linked promoter activities of gene regulatory elements in HEK293 cells

From: Multi-target regulatory mechanism of Yang Xin Tang − a traditional Chinese medicine against dementia

Promoter

% Luciferase activities changesa,b

Aqueous extract

Ethanol extract

YXT

Positive control

YXT

Positive control

Neurogenesis

 AP-1-RE

− 5.6 ± 0.1

66.3 ± 2.1c

36.8 ± 4.2c

118.7 ± 0.5c

 CRE

− 1.7 ± 2.6

96.9 ± 2.6c

15.6 ± 5.0c

153.2 ± 0.1c

 STAT3-RE

− 8.3 ± 1.9

56.7 ± 5.3c

− 11.8 ± 1.2

326.7 ± 2.9c

Neuronal cell survival

 LILRE

6.4 ± 1.4

30.8 ± 2.5c

− 3.1 ± 0.1

211.4 ± 2.7c

 p53-RE

− 6.9 ± 1.4

166.9 ± 5.2c

− 49.1 ± 0.1#

101.5 ± 7.8c

 TARE

− 11.7 ± 0.4

3612.2 ± 13.6c

− 23.2 ± 1.1#

2961.8 ± 57.6c

Aβ and NFT formation

 ISRE

− 4.2 ± 0.1

2504.0 ± 47.2c

4.1 ± 8.0

2753.9 ± 431.6c

 GAS

3.4 ± 2.5

29.8 ± 7.1c

− 17.1 ± 5.0d

18.6 ± 4.3c

 NFκB-RE

− 14.4 ± 1.8d

187.2 ± 8.5c

− 11.0 ± 0.5

149.8 ± 2.5c

  1. aChange in activity as compared to the corresponding basal. Data represent the mean ± S.E. of quadruplicate determinations in independent experiments (n = 4)
  2. bBasal activity (RFU): AP-1-RE and CRE: 10,500–30,000; LILRE, p53-RE, and TARE: 1000–5000; ISRE, and GAS and NFκB-RE: 100–500
  3. cSignificantly higher than the basal value (ANOVA and Dunnett’s test, P < 0.05)
  4. dSignificantly lower than the basal value (ANOVA and Dunnett’s test, P < 0.05)