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Table 2 Effects of the selected YXT herbal components on the activities of gene regulatory elements

From: Multi-target regulatory mechanism of Yang Xin Tang − a traditional Chinese medicine against dementia

Promoter

% Luciferase activity changes a,b

 

PA

PS

RA

RC

RP

Neurogenesis

 AP-1-RE

Aq

15.4 ± 0.4c

5.6 ± 1.0

− 1.0 ± 1.3

1.0 ± 0.4

− 6.2 ± 1.7

EtOH

− 6.4 ± 0.5

− 10.7 ± 0.4

34.2 ± 5.6c

− 11.9 ± 1.8

36.8 ± 0.2c

 CRE

Aq

− 3.5 ± 2.0

− 4.3 ± 1.9

− 6.5 ± 2.5

− 9.8 ± 1.1

− 0.9 ± 3.0

EtOH

20.9 ± 1.8c

0.8 ± 0.7

17.2 ± 2.8c

9.0 ± 2.3

34.8 ± 0.3c

 STAT3-RE

Aq

4.7 ± 5.2

− 12.2 ± 1.8

0.5 ± 1.8

− 6.1 ± 3.9

− 16.4 ± 3.2d

EtOH

− 2.2 ± 1.7

− 11.3 ± 1.1

12.7 ± 1.2

5.9 ± 2.7

0.6 ± 1.6

Neuronal cell survival

 LILRE

Aq

11.1 ± 0.5

4.8 ± 3.7

10.2 ± 1.0

7.8 ± 2.5

3.3 ± 0.2

EtOH

− 23.6 ± 0.6d

13.9 ± 3.4

2.0 ± 0.7

13.3 ± 2.7

0.4 ± 0.5

 p53-RE

Aq

7.7 ± 0.4

− 5.4 ± 3.0

2.3 ± 0.9

7.1 ± 0.9

1.9 ± 2.4

EtOH

− 55.5 ± 0.9d

− 31.1 ± 0.4d

− 11.3 ± 2.1

− 24.0 ± 1.3d

− 41.7 ± 1.8d

 TARE

Aq

− 21.7 ± 1.3

− 24.0 ± 3.8

− 3.2 ± 0.7

− 7.5 ± 0.4

− 2.3 ± 1.0

EtOH

− 14.1 ± 3.2

− 3.8 ± 6.8

− 25.3 ± 0.2d

− 8.4 ± 2.3

− 36.5 ± 1.7d

Aβ and NFT formation

 ISRE

Aq

19.7 ± 5.1

43.3 ± 16.6c

34.9 ± 11.7c

8.6 ± 4.6

69.4 ± 1.0c

EtOH

2.9 ± 1.6

− 16.0 ± 3.3d

− 5.0 ± 9.8

4.2 ± 2.1

30.3 ± 2.6c

 GAS

Aq

− 3.6 ± 0.5

8.2 ± 6.5

0.3 ± 2.2

− 3.3 ± 3.8

14.4 ± 12.1

EtOH

− 16.9 ± 1.0d

− 23.2 ± 1.7d

2.5 ± 4.9

− 18.4 ± 2.0d

− 3.6 ± 1.1

 NFκB-RE

Aq

− 13.8 ± 1.2d

− 17.7 ± 1.2d

− 4.2 ± 2.3

− 8.3 ± 2.4

− 14.3 ± 1.5d

EtOH

− 22.7 ± 1.4d

− 6.6 ± 3.5

21.6 ± 3.6c

− 7.6 ± 5.0

− 16.5 ± 0.9d

  1. Changes in luciferase activities induced by aqueous (Aq) and ethanol (EtOH) extracts are shown. Data are presented and analyzed as in Table 1 (n = 4)
  2. a,b,c,dPlease refer to Table 1 for details