Fig. 1

Curdione affects the activity of CT26 cells and causes changes in reactive oxygen species, Fe²⁺, MDA, and GSH. a. The cell viability of colorectal cancer CT26 cells was measured by MTT assay after 12.5 μM, 25 μM, and 50 μM curdione interventions for 48 h. b–f. The accumulation of reactive oxygen species was detected by flow cytometry after 12.5 μM, 25 μM, and 50 μM curdione interventions in CT26 cells for 48 h. g–i. Fe²⁺, MDA, and GSH levels in CT26 cells from different groups treated with curdione for 48 h were measured according to the manufacturer's instructions and ELISA kits. Values are expressed as the mean ± SD of three independent experiments. (n = 3) ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. control group