Fig. 4
From: Curdione induces ferroptosis mediated by m6A methylation via METTL14 and YTHDF2 in colorectal cancer
Curdione caused changes in ferroptosis and increased m6A modification in CRC xenograft mouse tissues, promoting m6A levels in SLC7A11 and HOXA13. a–c. ELISA kits for the determination of Fe2+, MDA, and GSH in tumor tissues of xenograft mice according to the manufacturer’s protocol. d. Spot blotting was used to determine the overall m6A levels of RNA in the four groups. The darker the color of the spot, the higher the m6A level. e–f. The effect of curdione on the m6A modification levels of SLC7A11 and HOXA13 was investigated using MeRIP-qPCR. Values are expressed as the mean ± SD of three independent experiments. (n = 3) *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group