Fig. 7

Curdione promoted ferroptosis in colorectal cancer through the upregulation of METTL14. a. The knockdown efficiency of METTL14 after 48 h of transfection of METTL14 shRNA CT26 cells was confirmed by Western blotting. ^**P < 0.01 vs. control group. b. Different concentrations (12.5 μM, 25 μM, and 50 μM) of curdione prepared from DMSO were added to CRC cells for 48 h. MTT assay for each group of transfected cell activities. ^##P < 0.01 H-CUR group vs. control group. ^**P < 0.01 H-CUR + shRNA-METTL14 group vs. H-CUR group. c–d. Flow cytometry for each group of transfected cell ROS. Curdione decreased the production of intracellular ROS. ^##P < 0.01 H-CUR group vs. control group. ^**P < 0.01 H-CUR + shRNA-METTL14 group vs. H-CUR group. e–g. ELISA kits for the determination of Fe²⁺, MDA, and GSH in transfected cells. ^**P < 0.01 H-CUR group vs. control group. ^##P < 0.01 H-CUR + shRNA-METTL14 group vs. H-CUR group. h–i. The protein levels of SLC7A11, SLC3A2, HOXA13, and GPX4 in METTL14 knockdown CRC cells were detected by Western blotting. GAPDH was used as a control. Values are expressed as the mean ± SD of three independent experiments. (n = 3) ^##P < 0.01 H-CUR group vs. control group. ^**P < 0.01 H-CUR + shRNA-METTL14 group vs. H-CUR group