Fig. 6

JGSQ mainly improves DR through the Akt/HIF-1α pathway. A-F Western blot was used to detect the effect of JGSQ on AKT, p-AKT, GAPDH, HIF-1α proteins, quantified by ImageJ software. Statistical analyses of IL-6, IL-1β and TNFα were performed using GraphPad Prism. G–I The expressions of p-AKT and HIF-1α were detected by immunofluorescence. (H–J) Statistical analyses of p-AKT and HIF-1α were performed using GraphPad Prism. Mock VS Model. ^nsP > 0.05, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001. JGSQ-treated VS Model. ^nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001