Table 1 The results of methodological validation
Compounds | Linearity (ug/mL) | r | LOD (ug/mL) | LOQ (ug/mL) | Precision (RSD%) | Stability (RSD%) | Repeatability (RSD%) | recovery (%) | recovery (RSD%) |
|---|---|---|---|---|---|---|---|---|---|
Coptisine | 0.57–57.25 | 0.9999 | 0.12 | 0.41 | 0.32 | 1.78 | 1.93 | 104.75 | 1.98 |
Epiberberine | 2.21–44.22 | 0.9999 | 0.15 | 0.48 | 0.40 | 0.92 | 2.95 | 100.94 | 4.67 |
Jatrorrhizine | 0.66–13.16 | 0.9999 | 0.10 | 0.33 | 0.38 | 1.80 | 1.54 | 101.57 | 1.95 |
Berberine | 0.86–86.24 | 0.9999 | 0.22 | 0.74 | 0.24 | 0.55 | 3.49 | 102.07 | 4.24 |
Palmatine | 2.05–41.14 | 0.9997 | 0.24 | 0.81 | 0.42 | 2.01 | 3.52 | 103.65 | 1.75 |
Baicalin | 2.34-234.32 | 0.9999 | 0.10 | 0.33 | 0.30 | 0.32 | 3.65 | 98.52 | 4.28 |
Wogonoside | 0.95–95.35 | 0.9999 | 0.07 | 0.23 | 0.26 | 0.79 | 1.49 | 100.37 | 1.83 |
Baicalein | 0.30-30.23 | 0.9993 | 0.09 | 0.29 | 0.59 | 5.67 | 3.50 | 98.29 | 3.46 |
Wogonin | 0.40–7.94 | 0.9999 | 0.06 | 0.20 | 0.23 | 1.24 | 2.75 | 96.21 | 4.32 |
Oroxylin A | 0.29–28.91 | 0.9999 | 0.09 | 0.29 | 0.45 | 1.50 | 2.69 | 95.32 | 0.78 |