Fig. 3

EGCG attenuated cell pyroptosis mediated by AIM2 through down-regulating MEG3 in OGD/R cells. A. Relative expression of MEG3 in OGD/R cells with and without EGCG treatment. B. Validation of the knockdown efficiency of shMEG3 in HL-1 cells. Cells were exposed to OGD/R before transfecting with shMEG3 or treating with EGCG (40 μM) and divided into six groups: control, OGD/R, OGD/R + shNC, OGD/R + shMEG3, OGD/R + shNC + EGCG, OGD/R + shMEG3 + EGCG. C. The measurement of MEG3 levels. D and E The analysis of cell viability and death (scale bar = 100 µm). F The measurement of LDH levels. G. Immunofluorescence assay was used to evaluate AIM2 levels. (scale bar = 100 µm). H Protein levels of AIM2, C-caspase-1, ASC, GSDMD-N, IL-18 and IL-1β. I. The expressions of IL-18 and IL-1β was detected by ELISA. The results were represented by three individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001