Fig. 2
PVE30 inactivated viral particles by interaction with viral glycoproteins. (A and B) HSV-1-infected cell lysates were incubated with heparin magnetic beads at 4 °C for 1 h, and 500 μg/mL PVE30 was then used to elute heparin-bound gB or gC at 4 °C for 1 h, followed by a Western blot assay to detect gB, gC, and gD expression (n=3). (C) HeLa cells infected with HSV-1/KOS at MOI=10 and treated with PVE30 during attachment and penetration. The immunofluorescence expression levels of viral gB, gC and gD were then detected using fluorescence microscopy. Scale bar = 20 μm. (D) HSV (2×105 PFU) was incubated with PVE30 (1 mg/mL) at 37 °C for 15 min and then infected into Vero cells at 1000-fold dilution. Infectivity was analysed by a plaque reduction assay (n=3)