Fig. 3

PVE30 suppressed HSV gene and protein expression and inhibits viral replication.  (A) Vero cells were infected with HSV-1/KOS or HSV-2/G at an MOI=0.1 for 1 h and treated with or without various concentrations of PVE30 (0.5×EC₅₀, 1×EC₅₀, and 2×EC₅₀). The viral mRNA expression levels (UL54, UL29, UL27, UL44, and US6) were determined at 3, 6, and 9 hours post infection by RT‒PCR (n=3). (B and C) Vero cells were infected with HSV-1/KOS or HSV-2/G at an MOI=1 for 1 h and treated with or without various concentrations of PVE30 (1, 2, 5, and 10 μg/mL). The viral protein expression levels (ICP27, ICP8, gB, gC, and gD) were determined at 3, 6, and 9 hours post infection by Western blot assay (n=3). (D) Vero cells were infected with HSV at an MOI=1 for an hour and treated with PVE30 (50 μg/mL). The infected cells and culture media were harvested at 0, 6, 12, 24 and 48 hours post infection and titered by a plaque reduction assay (n=3). (E) Vero cells were infected with HSV-1/KOS or HSV-2/G at an MOI=1 for 1 h and treated with or without various concentrations of PVE30 and ACV (1×EC₅₀ and 2×EC₅₀). The viral UL47 expression levels were determined at 24 hours post infection by RT‒PCR (n=3). *** P ≤ 0.001, ** P ≤ 0.01, * P ≤ 0.05, vs. virus control group