Fig. 4

PVE30 suppressed HSV-1-induced NF-κB activation. HeLa cells were infected with HSV-1/KOS at an MOI=1 for 1 h and treated with or without various concentrations of PVE30 (5, 10, 20, and 40 μg/mL) for 24 hours. (A and B) The protein expression levels (p-IKKβ, IKKβ, IκBα, p-p65, and p65) were determined by Western blot assay (n=3). (C and D) Cytoplasmic and nuclear proteins were extracted, and p65 nuclear translocation was determined by Western blot assay (n=3). (E) HeLa cells were infected with HSV-1/KOS at an MOI=1 for 1 h and treated with PVE30 (40 μg/mL) for 24 hours. The immunofluorescence expression of p-NF-κB was determined by fluorescence microscopy. Scale bar = 20 μm. (F) The mRNA expression level of NF-κB was determined by RT‒PCR (n=3). *** P ≤ 0.001, ** P ≤ 0.01, * P ≤ 0.05, vs. virus control group