Fig. 5

PVE30 suppressed HSV-1-induced activation of TLR pathway. HeLa cells were infected with HSV-1/KOS at an MOI=1 for 1 h and treated with or without various concentrations of PVE30 (5, 10, 20, and 40 μg/mL) for 24 hours. (A) The mRNA expression levels of TNFα and IL-6 were determined by RT‒PCR (n=3). (B) The mRNA expression levels of TLR2, TLR3, and TLR4 were determined by RT‒PCR (n=3). (C and D) The protein expression levels of MyD88 and TRAF6 were determined by Western blot assay (n=3). *** P ≤ 0.001, ** P ≤ 0.01, * P ≤ 0.05, vs. virus control group