Fig. 6

PVE30 suppressed HSV-1-induced necroptosis. HeLa cells were infected with HSV-1/KOS at an MOI=1 for 1 h and treated with or without various concentrations of PVE30 (10, 20, and 40 μg/mL) for 24 hours. (A and B) The cells were harvested and incubated with an Annexin V-FITC/PI Apoptosis Detection Kit and then analysed using CytExpert software. LL (lower left) represents viable cells (%), UL (upper left) represents necroptotic cells (%), LR (lower right) represents early apoptotic cells (%), UR (upper right) represents late apoptotic cells (%), (n=3). (C and D) The protein expression levels of p-MLKL and MLKL were determined by Western blot assay (n=3). *** P ≤ 0.001, ** P ≤ 0.01, * P ≤ 0.05, vs. virus control group