Table 1 The primer sequences used in real-time PCR
Genes | Primer sequence (5′-3′) | |
|---|---|---|
Monkey 18S | Forward | ACACGGACAGGATTGACAGATTGATAG |
Reverse | ACCAGACAAATCGCTCCACCAAC | |
HSV-1 UL54 | Forward | TGTCGGGGTCGGAGAGAAGATG |
Reverse | GGCGGTGCGTGTCTAGGATTTC | |
HSV-2 UL54 | Forward | TTGCCTCCTTTGTGTTGGTCATCC |
Reverse | TTTCAATGAGACCCGCCATGCAG | |
HSV-1 UL29 | Forward | AGGCTCGGACAAGGTAACCATAGG |
Reverse | TTGAACGGCTCTGCGATGACAC | |
HSV-2 UL29 | Forward | TGTTTGTGGCGACCGTCAAGAG |
Reverse | CTCAGGTACTCGTCCTCCAGCAG | |
HSV-1 US6 | Forward | ACACCGAATGCTCCTACAACAAGTC |
Reverse | CGCTGAAGCTGTCATAGTAGTTCCAG | |
HSV-2 US6 | Forward | TCTCAGACTCACTCGTGGATGCC |
Reverse | TGATGCCGTCGTAGTAGTTTGTGTG | |
HSV-1 UL27 | Forward | CATATCCACCACCTTCACCACCAAC |
Reverse | GTTGTACCTGCGGGCGAAGATG | |
HSV-2 UL27 | Forward | CACACCACCGACCTCAAGTACAAC |
Reverse | TCGCCAGCACAAACTCATCGTAC | |
HSV-1 UL44 | Forward | TAAAGCCGCCACCCTCTCTTCC |
Reverse | TGCCGTTGTGTTGGTAGGAAAGC | |
HSV-2 UL44 | Forward | TACTGGTGGGTGAACGGAGACG |
Reverse | TCGAAGTTACGAAGCGGACAAACC | |
HSV-1 UL47 | Forward | CCATCACCACGCCCAGTATATCATC |
Reverse | GCCCGCAGATACTCGTTGTTCAG | |
HSV-2 UL47 | Forward | ACCGTCGAGGTTCTATCCATAGTCC |
Reverse | GTAGGTACTCGTTGTTCAGGCTGTC | |
Human 18S | Forward | GGACACGGACAGGATTGACAGATTG |
Reverse | TAACCAGACAAATCGCTCCACCAAC | |
Human TNFα | Forward | CCTCATCTACTCCCAGGTCCTCTTC |
Reverse | TCTGGTAGGAGACGGCGATGC | |
Human IL-6 | Forward | ACTCACCTCTTCAGAACGAATTG |
Reverse | CCATCTTTGGAAGGTTCAGGTTG | |
Human NF-κB | Forward | CCCACGAGCTTGTAGGAAAGG |
Reverse | GGATTCCCAGGTTCTGGAAAC | |
Human TLR2 | Forward | TTGGTCACCGTGGCTCAGAGG |
Reverse | AGCAGAGGTCAGACTTGGGTTAGG | |
Human TLR3 | Forward | CCTTTACTCCTCTGGCGGTTTCG |
Reverse | CTTGGCTGTTCTGGGACTCACTTC | |
Human TLR4 | Forward | GTGAGGATGATGCCAGGATGATGTC |
Reverse | TGACTCCAGCCACATACCTCCAC | |