Fig. 6

Treatment of T0 and MGF activates AMPK signaling pathway to suppress hepatic lipogenesis and induce autophagy to accelerate hepatic degradation in Apoe^−/− mice. A Expression of p-AMPK, AMPK, and CD36 was determined by western blot with total proteins extracted from liver samples of Apoe^−/− mice. B Expression of lipid biosynthesis related SREBP-1c, p-ACC, ACC, FAS and SCD-1. C Expression of lipolysis related ATGL, p-HSL and HSL, and β-oxidation relative CPT1. D Expression of autophagy related LC3II, ATG5, ATG7, p-p38 and p38. ^***p < 0.001, ^**p < 0.01, ^*p < 0.05 (n = 3). E Hepatic red fluorescent LC3 protein was determined by immunofluorescent staining. Scale bar, 50 μm