Table 1 MRM parameters for each compound and internal standard to be tested in the PK study
Compounds | Ion Mode | Rt (min) | Precursor (m/z) | Product ion (m/z) | Q1 (V) | Collision energy (V) | Q3 (V) |
|---|---|---|---|---|---|---|---|
Ephedrine | + | 1.7 | 166.2 | 148.2 | 14 | 15 | 15 |
Methylephedrine | + | 1.9 | 180.1 | 162.2 | 11 | 15 | 30 |
Songorine | + | 1.9 | 358.1 | 340.1 | 20 | 30 | 20 |
Fuziline | + | 2.4 | 454.2 | 436.1 | 12 | 33 | 21 |
Neoline | + | 2.6 | 438.2 | 420.2 | 10 | 30 | 21 |
Albiflorin | + | 3.0 | 480.9 | 105.2 | 23 | 25 | 21 |
Talatizamine | + | 3.3 | 422.3 | 390.2 | 15 | 30 | 29 |
Calycosin-7-glucoside | + | 4.2 | 446.9 | 285.1 | 21 | 21 | 20 |
Benzoylmesaconine | + | 7.3 | 590.1 | 105.1 | 28 | 53 | 20 |
Benzoylaconine | + | 8.3 | 604.4 | 105.0 | 22 | 54 | 19 |
Benzoylhypaconine | + | 8.9 | 574.2 | 542.2 | 20 | 36 | 20 |
Reserpine | + | 10.1 | 609.3 | 195.1 | 22 | 37 | 21 |
Oxypaeoniflorin | − | 1.7 | 495.1 | 137.2 | 23 | 30 | 12 |
Paeoniflorin | − | 2.6 | 479.1 | 448.9 | 23 | 10 | 22 |
Liquiritin apioside | − | 2.9 | 549.3 | 255.2 | 24 | 32 | 11 |
Liquiritin | − | 3.0 | 417.2 | 255.1 | 18 | 22 | 11 |
Naringin | − | 3.3 | 579.3 | 271.1 | 24 | 35 | 17 |
Isoliquiritin | − | 3.8 | 417.2 | 255.1 | 18 | 19 | 11 |
Liquiritigenin | − | 4.3 | 255.2 | 119.1 | 12 | 24 | 11 |
Calycosin | − | 4.5 | 283.1 | 211.2 | 29 | 34 | 20 |
Benzoylpaeoniflorin | − | 4.9 | 583.1 | 431.0 | 40 | 17 | 19 |
Isoliquiritigenin | − | 5.8 | 255.3 | 119.1 | 16 | 24 | 11 |
Glycyrrhizic acid | − | 5.9 | 821.6 | 351.0 | 20 | 40 | 11 |
Formononetin | − | 6.0 | 267.0 | 223.2 | 13 | 32 | 21 |
Glycyrrhetinic acid | − | 9.1 | 469.5 | 425.4 | 12 | 40 | 20 |