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Table 2 Standard curves and linear ranges for each compound to be tested in PK studies

From: Regulation of polysaccharide in Wu‐tou decoction on intestinal microflora and pharmacokinetics of small molecular compounds in AIA rats

Analytes

Calibration curve

r2

Linear range (ng/mL)

Ephedrine

y = 0.8825x + 3.7555

0.9928

10–1000

Methylephedrine

y = 1.5091x + 0.0095

0.9945

0.1–25

Songorine

y = 2.1609x-0.0359

0.9905

0.1–50

Fuziline

y = 1.4974x-0.0152

0.9915

0.1–25

Neoline

y = 0.4646x-0.0020

0.9923

0.1–50

Albiflorin

y = 0.1032x + 0.0452

0.9916

5–500

Talatizamine

y = 1.9382x-0.0038

0.9928

0.1–50

Calycosin-7-glucoside

y = 0.1515x-0.0034

0.9936

0.1–100

Benzoylmesaconine

y = 1.0726x-0.0059

0.9929

0.1–100

Benzoylaconine

y = 0.7982x + 0.0047

0.9962

0.05–10

Benzoylhypaconine

y = 1.5379x-0.0135

0.9973

0.1–10

Oxypaeoniflorin

y = 0.9149x-0.0075

0.9995

0.5–50

Paeoniflorin

y = 0.0324x + 0.0288

0.9938

2.5–2500

Liquiritin apioside

y = 2.8756x-0.1369

0.9985

1–2500

Liquiritin

y = 4.1916x + 0.1199

0.9961

1–500

Isoliquiritin

y = 3.6659x-0.0129

0.9999

0.5–50

Liquiritigenin

y = 2.9112x + 0.0522

0.9990

0.5–100

Calycosin

y = 9.7214x-0.0172

0.9958

0.1–25

Benzoylpaeoniflorin

y = 0.0862x-0.0007

0.9992

1–50

Isoliquiritigenin

y = 6.5489x-0.0406

0.9993

0.5–50

Glycyrrhizic acid

y = 0.6790x-0.3716

0.9982

100–10000

Formononetin

y = 13.0676x + 0.0165

0.9974

0.5–50

Glycyrrhetinic acid

y = 3.2768x + 0.3358

0.9948

100–20000