Fig. 3
S.C-induced cell death depends on ferroptosis. PC3 a and DU145 b cells were treated with S.C (0.5 μM) with or without Z-VAD-FMK (20 μM), fer-1 (2.0 μM), and DFO (5.0 μM) for 48 h. Then, Cell survival rates were measured using CCK8 assay. PC3 c, e and DU145 d, f cells were treated with S.C (0.5, 1.0 μM) for 48 h, intracellular ROS, ferrous ion (Fe2+), MDA g and GSH-PX h level were detected. i, j Western blotting analysis of SLC7A11 and GPX4 levels in PC3 and DU145 cells receiving S.C treatment or not for 48 h. k, l TEM images of the mitochondrial structure in PC3 and DU145 cells treated with or without S.C (0.5 μM)for 24 h