Fig. 5

S.C inducing HMOX-1 up-regulation contributes to ferroptosis in prostate cancer cell. mRNA and protein levels of HMOX1 measured by qRT-PCR and western blot in PC3 HMOX1 knockdown a, c and DU145 HMOX1 knockdown cells b, d. DCFH-DA probe was used to detected ROS levels in PC3 HMOX1 knockdown e and DU145 HMOX1 knockdown f cells with S.C treatment or not. Mito-FerroGreen probe was used to detected iron levels in PC3 HMOX1 knockdown g and DU145 HMOX1 knockdown (h) cells. i, j Quantification of the MDA levels in HMOX1 knockdown cells. GPX4 and SLC7A11 protein level in PC3 HMOX1 knockdown k and DU145 HMOX1 knockdown l cells