Fig. 6

S.C induces HMOX1 up-regulation depending on decreasing the stability of BACH1 protein. Western blotting analysis of PERK, STAT3, NRF2, BACH1, KEAP1, and HIF-1α levels in PC3 a and DU145 b cells receiving S.C (0, 0.5, 1.0 μM) treatment for 48 h. Immunoblotting analysis of BACH1 in nuclear and cytoplasm of S.C (0, 0.5, 1.0 μM)-treated PC3 c and DU145 d cells. e mRNA expression level of BACH1 in S.C-treated PC3 and DU145 cells. f–g Cycloheximide (CHX) chase assay in S.C-treated DU145 cells. h BACH1 protein levels were calculated by quantification of BACH1protein levels. Immunoblotting analysis of USP47 in S.C (0, 0.5, 1.0 μM)-treated PC3 i and DU145 j cells. Immunoblotting analysis of BACH1 and HMOX1 in S.C (0, 1.0 μM)-treated PC3 k and DU145 l cells transiently expressing empty vector or BACH1 vector