Fig. 7

ROS is responsible for BACH1 decreasing in S.C inducing ferroptosis. Cells were pretreated with vehicle or 5 mM NAC for 1 h before treatment with 0.5 μM S.C in PC3 a, b, g, h or DU145 c, d, i, j cells. After 24 h treatment with vehicle or S.C in the presence of NAC, cells were stained with DCFH-DA or Mito-FerroGreen for flow cytometry assay. After 48 h treatment with vehicle or S.C in the presence of NAC, cells were used to analyze the contents of GSH, MDA in PC3 e, k and DU145 cells f, l. After 48 h treatment, GPX4, SLC7A11, HMOX1, BACH1 and USP47 levels were analyzed by Immunoblotting in PC3 m and DU145 cells n