Fig. 6

Regulatory effects of Osteoking on the expression of the corresponding proteins in ZBP1-STAT1-PKR-MLKL signal axis in bone tissues of bone defect rats. A The expression levels of p-STAT1 protein were measured by immunofluorescence staining and confocal microscopy in bone tissue sections (p-STAT1 green, DAPI blue; Original magnification, ×400; scale bar, 50 μm). B The expression levels of ZBP1 and p-PKR/PKR proteins in bone tissues of bone defect rats using Western botting analysis (n = 3 per group). C Mean density of immunofluorescence staining of p-STAT1 protein expression in the bone tissues of different groups. D The expression levels of ZBP1 in bone tissue supernatants were measured by Western blotting. E The expression levels of p-PKR in bone tissue supernatants were measured by Western blotting. F–H The expression levels of inflammatory factors in serum were detected by ELISA. F Tumor necrosis factor (TNF-α; pg/mL). G Interleukin-1β (IL-1β; pg/mL). H Interleukin-6 (IL-6; pg/mL). Bars show mean ± SD; ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 indicate the significance compared to the normal control group, respectively; *P < 0.05, **P < 0.01 and ***P < 0.001 indicate the significance compared to the model group, respectively